Protein Assays
AURORA BIOLABS LLC TEV Protease-GST tag (Ready-to-Use), 50 ug
Tobacco Etch Virus nuclear-inclusion-a endopeptidase or TEV is highly sequence specific cysteine protease with N-terminal GST tag. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln↓(Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins. Purified TEV was expressed in E. coli and is shipped in 4°C packaging to preserve activity. TEV cleavage is best performed at room temperature in a pH range of 4 – 9.
Abcam ATP synthase Enzyme Activity Microplate Assay Kit
ATP synthase Enzyme Activity Microplate Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Revvity Health Sciences Inc HTRF EPIgeneous 3-methyl K27 Histone H3 Detection Kit, Control Lysate
HTRF EPIgeneous 3-methyl K27 Histone H3 Detection Kit, Control Lysate
Cayman Chemical Hydroxybutyrate Dehydrogenase
A SERT DAT and NET inhibitor inhibits serotonin dopamine and norepinephrine uptake in HEK293 cells expressing human SERT DAT and NET (IC50s 8 48.2 and 17 UM respectively)
Bioassay Systems EnzyChrom™ Kinase Assay Kit. Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors.
Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day. Method: FL530/585nm. Samples: All kinases. Species: All. Procedure: Assay takes 10 min. Kit size: 400 tests. Detection limit: 0.01 U/L.
Bioassay Systems EnzyChrom™ Phospholipase D Assay Kit. For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism. Key Features: Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L. Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.04 U/L.
Bioassay Systems QuantiChrom™ Β-N-Acetylglucosaminidase Assay Kit.Β-N-Acetylglucosaminidase activity determination in biological samples.
For quantitatve determination of β-N-Acetylglucosaminidase activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 50 U/L for a 30 minute reaction at 37°C. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Urine, serum, plasma, cell lysate, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.2 U/L.